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Sell: Recombinant Enterokinase |
| Date: |
2017-02-22 |
| Cas#: |
9017-74-8 |
| Specification: |
100U |
| Price: |
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| Quantity: |
IU |
| Term: |
365 |
| Note: |
Recombinant Enterokinase
Catalog Number:REK08
CAS:9017-74-8
EC:3.4.21.9
Source: bovine enterokinase, expressed in E. Coli
DESCRIPTION
YaxinBio Enterokinase is a kind of highly purified recombinant bovine enterokinase. The enzyme has been extensively purified and there are no traces of other contaminating proteases. Enterokinase specifically hydrolyzes peptide bond at the carboxyl side of lysine residue preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK). So, Enterokinase can remove N-terminal fusion protein or tags to get aim protein with native amino acids sequence.
ADVANTAGES
1) Protease that cleaves specifically after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys (DDDDK)
2) No any other contaminated proteases, no non-specific cutting sites.
Recommend Usage Condition:
Cutting condition: 25mM Tris-HCl 8.0
Fusion protein concentration: 0.1-1mg/ml (total protein content: 0.5-1.0mg)
EK content: 1-2U
Temperature: 25芒鈥炂抃
Time: overnight or 12h-16h for digestion.
If digesttion under 2芒鈥炂?8芒鈥炂?prolong the digestion time two folds,or more enzyme added to short the digestion time.
Common components influence the action of enterokinase
茂录啪200mM imidazole or 茂录啪200mM NaCl or 茂录啪5% glycerin, the reaction may be effected.The following suggestions are given:
1) To receive the optimum result, please dialyze the sample to 25 mMTris-HCl, pH 8.0.
2) If the dialysis is inconvenient, please dilute the sample to 茂录艙100mM imidazole, 茂录艙50mMNaCl, 茂录艙5% glycerin, and the proportion of fusion protein and EK may not be changed (1U:0.5mg fusion protein).
3) If there are one or more components in samples, and cannot be removed, suggest to increase the content of EK in reaction system or extend the reaction time.
MAIN FEATURES
Source E.Coli
M.W. 25,850 Da 脗聽
Specific Activity One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25脗掳C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.脗聽
Storage Temperature -20脗掳C or below. 脗聽
Stability Keep cool with blue ice during shipping. Remained stable at 25脗掳C for one week without activity lost. No activity lost after 5 cycles of frozen-thawing.
PRODUCT INFORMATION
Product Activity Packaging Manufacturer
Recombinant Enterokinase 芒鈥奥? u/脦录l 100u;500u;bulk YaxinBio |
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