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Sell: Recombinant Proteinase K |
| Date: |
2017-02-22 |
| Cas#: |
39450-01-6 |
| Specification: |
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| Price: |
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| Quantity: |
100000Kilogram |
| Term: |
365 |
| Note: |
Recombinant Proteinase K
Catalog Number:RPK09
Cas:39450-01-6
EC:3.4.21.14
Alternate names:Protease K, Endopeptidase K, Tritirachium alkaline proteinas
DESCRIPTION
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
ADVANTAGES
Format Lyophilized
M.W 29.8kD
Purity 99%
Activity 30 units/mg
DNAses and RNAses not detected
UNIT DEFINITION
One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37脗掳C, pH 7.5(color by Folin-Ciocalteu reagent).
RECOMMEND USAGE
Prepare recombinant Proteinase K with 50 mM Tris-HCl,2 mM Calcium acetate.The optimum pH is 8.0.
STORAGE INSTRUCTIONS
Store lyophilized at -20脗掳C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.
HANDLING
Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure. |
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