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荧光素 |
| CAS No.: |
2321-07-5 |
| 分子式： |
C20H12O5 |
| 分子量： |
332.31 |
| 备注： |
中文名称荧光素中文同义词荧光橙红;荧光素,指示剂;黄7[CI45350:1];荧光素,水溶;荧光酯;萤光橙黄;萤光红;萤光黄英文名称Fluorescein英文同义词FLUORESCEIN,FREEACIDFLUORESCEIN,FREEACIDFLUORESCEIN,FREEACID;Diresorcinolphthalein;Tetraoxyphthalophenoneanhydride;SOLVENTYELLOW94;SNO880;RESORCINOLPHTHALEIN;FLUORESCEIN,ALCOHOLSOLUBLE;FLUORESCEINCAS号2321-07-5分子式C20H12O5分子量332.31EINECS号219-031-8相关类别滴定;分析试剂;标准品;色素类;生化试剂;生物染色剂;小分子抑制剂;基因组学和分子诊断;化合物;生化试剂-色素类;化学试剂;化工原料;大化工;E-FFluorescentProbes,Labels,ParticlesandStains;FluoresceinandDerivatives;FluorescentLabels;StainsandDyes;Stains&Dyes,Ato;Aromatics;FluorescentLabels&Indicators;Heterocycles;Intermediates&FineChemicals;Pharmaceuticals;FUNDUSCEIN;Fluorescent;Xanthene;化工材料;有机试剂;通用生化试剂-生物染色剂与化学指示剂;染料;试剂盒;日化品原料;有机化工原料Mol文件2321-07-5.mol结构式荧光素性质熔点320°C(lit.)沸点429.44°C(roughestimate)密度1.2739(roughestimate)折射率1.5000(estimate)储存条件roomtemp溶解度不溶于水、乙醚、苯、氯仿；溶于乙醇、甲醇、丙酮、乙酸乙酯、N,N-二甲基甲酰胺酸度系数(pKa)2.2,4.4,6.7(at25℃)色指数45350形态粉末颜色红色到橙色酸碱指示剂变色ph值范围Pinkuorescence(4.0)togreenuorescence(6.0)水溶解性insoluble最大波长(λmax)493.5nm,496nm,460nm,515nmMerck14,4159BRN94324稳定性稳定的。易燃。与强氧化剂不相容。主要应用Organiclight-emittingdiode,nanoparticles,liquidcrystaldisplay,oilproducts,lipmake-up,nucleicacidsynthesis,amplification,sequencingandcloning,diagnosisofdiabeticretinopathy,detectingyeast,multidrugresistance,proteins,antiHCVantibodies,targetgenes,enzymaticactivity,nerveagent,nucleicacidsequences,imaginglungcancer,prostatecancer生物领域应用Detectingchromosomalaberration;diagnosingcornealdiseases;marking/detectinginsects;asantitumoragent;asantihistaminicagent;useinagricultureandplantcultivation;useincosmetics;useinophthalmology;asasubstrateformeasuringα-amylasesactivity,elastasesactivity,esterasesactivity,βChemicalbook-glucuronidasesactivity,horseradishperoxidasesactivity,kinasesactivity,monoamineoxidases(MAOs)activity,phosphatasesactivity,phospholipasesactivity,proteases/proteinasesactivity,telomerasesactivityactivityLogP2.980(est)CAS数据库2321-07-5(CASDataBaseReference)EPA化学物质信息Fluorescein(2321-07-5)荧光素用途与合成方法生物活性Fluorescein(Resorcinolphthalein)是一种合成的有机光敏染料化合物。Fluorescein是荧光示踪剂，可以应用于医学和生物学以及肿瘤感染的组织。体外研究Fluoresceinisnearlyinactiveasaphotodynamicsensitizer.Fluoresceinconsequentlyisalmostuselessasaphotodynamicsensitizer,butitisofgreatvalueinfluorescenceimmunoassays.Thefluoresceindyeisacommonfluorescentprobe.Itsveryhighmolarabsorptivityatthewavelengthoftheargonlaser(488nm),largefluorescencequantumyieldandhighphotostabilitymakesitaveryusefulandsensitivefluorescentlabel.Fluoresceiniscommerciallyavailableinmanyderivatives,suchasfluoresceinisothiocyanateandfluoresceinsuccinimidylester,thatcanbecovalentlyattachedtomacromoleculesandtoaminoacids.Thelabeledmoleculescanbedetectedwithveryhighsensitivitywhichisutilizedin,forexample,capillaryelectrophoresis.Theemissionspectrumoffluoresceinoverlapsextensivelytheabsorptionspectrumoftetramethylrhodamine,whichisarelatedstronglyfluorescentdye,makingthispairverysuitableforenergytransferexperimentstodeterminedistanceswithinandbetweenlabeledmacromolecules.Fluoresceininaqueoussolutionoccursincationic,neutral,anionicanddianionicformsmakingitsabsorptionandfluoresencepropertiesstronglypHdependent.化学性质荧光素外观呈橙黄或浅橙红色结晶粉末。熔点314-316℃（分解）。溶于热乙醇、冰醋酸、碱溶液，呈绿色萤光，不溶于水、苯、氯仿和乙醚。用途荧光素是汞溴红的中间体，可用作萤光光度分析试剂，测定氯、溴、碘、硫离子；氧化-还原指示试剂；生物染色剂。用途荧光光度分析硫离子，试验溴，滴定氯、溴和碘。吸附指示剂，氧化还原指示剂。用途吸附指示剂；氧化还原指示剂；荧光光度分析硫离子；滴定氯、溴和碘生产方法荧光素可用邻苯二甲酸酐与间苯二酚在硫酸参与下反应制得。 |
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