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异硫氰酸荧光素酯 |
| CAS No.: |
3326-32-7 |
| 分子式： |
C21H11NO5S |
| 分子量： |
389.38 |
| 备注： |
中文名称异硫氰酸荧光素酯中文同义词异硫氰酸荧光酯异构体/FITC;FITC,荧光素-5-异硫氰酸盐异构体I;荧光胺异硫腈酸盐;荧光素-5-异硫氰酸盐异构体I;5-异硫氰酸荧光素酯;5-异硫氰酸荧光素;异硫氰酸荧光素(异构体I);异硫氰酸荧光素,ISOMER1,95%英文名称FluoresceinisothiocyanateisomerI英文同义词FITC;FITC-CELITE(R);FITCISOMER;FITC’ISOMERI’;FITC,ISOMERI;FLUORESCEINISOMERIISOTHIOCYANATE;FLUORESCEINISOTHIOCYANATE,ISOMER1;FLUORESCEINISOTHIOCYANATEISOMERICAS号3326-32-7分子式C21H11NO5S分子量389.38EINECS号222-042-0相关类别蛋白质组学;荧光探针，标记，颗粒和染色;荧光标记染料;生化试剂;色素类;荧光素类，荧光染料;化学发光;其他生化试剂;化学试剂;生化试剂-色素类;分析试剂-显色剂;生物化工;高纯生化试剂;Phenylisocyanate&Phenylisothiocyanate;FluorescentLabelsandIndicators;AminoGroupLabelingReagentsforFluorescenceHPLC;AnalyticalChemistry;FluorescenceDetection(HPLCLabelingReagents);HPLCLabelingReagents;FluorescentLabels&Indicators;marker;Fluorescent;Xanthene;通用生化试剂-生物染色剂与化学指示剂;化工中间体;有机化工原料;荧光染料Mol文件3326-32-7.mol结构式异硫氰酸荧光素酯性质熔点>360°C(lit.)沸点708.6±60.0°C(Predicted)密度1.3423(roughestimate)折射率1.5060(estimate)储存条件2-8°C溶解度微溶于DMSO、乙醇、甲醇DMF和丙酮酸度系数(pKa)2.2,4.4,6.7(at25℃)形态粉末颜色白色酸碱指示剂变色ph值范围Weakgreenuorescence(6.0)tostronggreenuorescence(7.2)水溶解性practicallyinsoluble最大波长(λmax)490nm敏感性MoistureSensitiveBRN1407295稳定性稳定，但对水分敏感。与强氧化剂、强碱、胺、酸不相容。主要应用Magneticnanowires,immunoassays,analyzingproteins,identifyingchromosomes,Salmonellamosomes,diagnosisofcancer,kidneydiseases,detectingpathogens,genes,Salmonellacells,toxoplasmosis,enzyme-mediatednucleicacidcleavage,surfacemoleculesoncolorectalcaChemicalbookncers,treatingcancer,chroniclymphocyticleukemia生物领域应用Analyzingsugarchain;detectingiodide/iodateortotaliodine;detecting/imagingthiols;detecting/quantifyingbiotinderivatives;16diagnosingclassicalHodgkinlymphoma(CHL)inlymphnodes;cellulosenanocrystalsforbioimagingapplications;treatingneuroinflammation;treating/diagnosingcancer;PEO-PCL-PEOtriblockcopolymersfortopicaldelivery;polymersomesinbiomedicalorcosmeticapplications;apoptosisassay;asasubstrateformeasuringheparanaseactivity,humanimmunodeficiencyvirus(HIV-1)proteinaseactivity,kinaseactivity,phosphataseactivity,β-lactamaseactivity,transglutaminase(TG2)activity;implantabledrug-deliverydevicessInChIKeyMHMNJMPURVTYEJ-UHFFFAOYSA-NCAS数据库3326-32-7(CASDataBaseReference)EPA化学物质信息Spiro[isobenzofuran-1(3H),9’-[9H]xanthen]-3-one,3’,6’-dihydroxy-5-isothiocyanato-(3326-32-7)异硫氰酸荧光素酯用途与合成方法简介异硫氰酸荧光素酯是黄色粉末，有吸湿性。其能与各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中仍有强烈绿色荧光，加酸后析出沉淀，荧光消失，微溶于丙酮、乙醚和石油醚。用途异硫氰酸荧光素酯能和各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，并在碱性溶液中具有强烈的黄绿色荧光。通过在荧光显微镜下观察或流式细胞仪分析可对相应抗原进行定性、定位或定量的检测。用于医学，农学和畜牧等方面，可对由细菌病毒和寄生虫等所致疾病进行快速诊断。生物活性FITC(Fluorescein5-isothiocyanate)是一种用于胺标记的荧光探针。FITC是对pH和Cu2+敏感的荧光染料。体外研究AnnexinV-FITC/PIisemployedtodetectchondrocyteapoptosis.ThePEI-modifiedCDsareconjugatedwithfluoresceinisothiocyanate(FITC)tofabricateCDs-FITCcomposites.用途蛋白质荧光标记试剂。用于荧光抗体技术快速鉴定病原体。染料和代谢物。用途生化研究。荧光抗体示踪。病毒和寄生虫等所致疾病的快速诊断。 |
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