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2-硝基苯-beta-D-半乳糖苷 |
| CAS No.: |
369-07-3 |
| 分子式： |
C12H15NO8 |
| 分子量： |
301.25 |
| 备注： |
中文名称2-硝基苯-beta-D-半乳糖苷中文同义词2-硝基苯基-~-D-半乳糖苷,98+%;邻硝基苯-Α-D-吡喃葡萄糖苷/2-硝基苯-Α-D-吡喃葡萄糖苷/ONPG;2-硝基苯基-Β-D-吡喃半乳糖苷(动物源);邻硝基苯-BETA-D-半乳糖苷;2-硝基苯基-Β-D-吡喃半乳糖苷(植物源);2-硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Β-D-半乳糖苷(ONPG)英文名称2-Nitrophenyl-beta-D-galactopyranoside英文同义词2-Nitrophenylhexopyranoside;2-Nitrophenyl-beta-delta-galactopyranoside;2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol;o-Nitrophenyl-β-D-galactopyranoside-CAS369-07-3-Calbiochem;beta-D-Galactopyranoside,2-nitrophenyl;beta-d-galactopyranoside,2-nitrophenyl;Galactopyranoside,o-nitrophenyl,beta-D-;o-Nitrophenylbeta-galactosideCAS号369-07-3分子式C12H15NO8分子量301.25EINECS号206-716-1相关类别化学试剂;分子生物试剂-多肽与蛋白;碳水化合物;专业试剂;酶类;中间体;氨基酸;核苷;酶底物;糖类化合物;糖类;检测标志试剂;即用型溶液;糖类（碳水化合物）;Substrates;Biochemistry;Galactose;Glycosides;Sugars;Enzymesubstrates;Carbohydrates&Derivatives;生化试剂;substrate;糖苷系列;多肽与蛋白;酶活性检测;酶及活性检测;试剂;优势产品;生化试剂-酶类;其他原料;精细化工原料;化学原料;通用生化试剂-酶与辅酶;还原剂;化工中间体;其它;化学试剂Mol文件369-07-3.mol结构式2-硝基苯-beta-D-半乳糖苷性质熔点186-193°C(dec.)比旋光度-70º(c=1,H2O)沸点442.39°C(roughestimate)密度1.3709(roughestimate)折射率-68.8°(C=1,H2O)储存条件2-8°C溶解度DMSO（微溶）、水（微溶、加热、超声处理）酸度系数(pKa)12.69±0.70(Predicted)形态粉末颜色白色至微Chemicalbook黄色旋光性(opticalactivity)[α]20/D70±1°,c=1%inH2O水溶解性Solubleinwater.BRN92207InChIKeyKUWPCJHYPSUOFW-RMPHRYRLSA-NCAS数据库369-07-3(CASDataBaseReference)NIST化学物质信息Galactopyranoside,o-nitrophenyl,beta-d-(369-07-3)2-硝基苯-beta-D-半乳糖苷用途与合成方法生物活性ONPG是一种比色和分光光度计底物，用于检测β-半乳糖苷酶活性。体外研究TheenzymedisplayshighhydrolysisabilityforONPG(100%)andmoderateactivityforitsnaturalsubstratelactose(25.7%).However,thehydrolysisabilityoftheenzymetowardsallotherchromogenicnitrophenylanaloguesisveryweak,indicatingthatGal308isaβ-galactosidasewithnarrowsubstratespecificity.Toinvestigatethekineticparametersofrecombinantenzyme,theMichaelis-Mentenconstants(Km),turnovernumbers(kcat),andcatalyticefficiencies(kcat/Km)ofGal308forONPGandlactosearedetermined.ThekcatandKmvaluesare464.7±7.8s-1and2.7±0.3mMforONPG,and264.2±2.1s-1and7.1±0.8mMforlactose,respectively.Thekcat/KmvalueoftheenzymeforONPG(172.1s-1mM-1)is4.6-foldhigherthanthatforlactose(37.2s-1mM-1),whichclearlydemonstratedthatthecatalyticefficiencyofGal308forONPGismuchhigherthanthatforlactose.用途用于测定β-半乳糖苷酶活性用途生化研究用途用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。 |
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