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双链聚肌胞 |
| CAS No.: |
42424-50-0 |
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中文名称双链聚肌胞中文同义词聚肌苷酸-聚胞苷酸钠盐;双链聚肌胞;6.双链聚肌胞(POLYI:C.);卡那霉素-聚肌胞钠盐复合物;双链聚肌胞钠盐;双链聚肌胞溶液;POLYI:C双链聚肌胞钠盐;聚肌苷-聚胞苷酸钠盐英文名称Polyinosinic-polycytidylicacidsodiumsalt英文同义词POLYINOSINICACID+POLYCYTIDYLICACID,SODIUMSALT;Polymyocytesodiumsalt;POLYINOSINICACIDPOLYCYTIDYLICACID,SODIUMSALT;POLYINOSINIC-POLYCYTIDYLICACIDSODIUMSALT;POLYIPOLYC,NA;POLY[I]POLY[C]SODIUMSALT;Oligochitosan-kanamycin-polyI-polyCsodiumsaltcompoundsolution;Polyinosinicacid-polycytidylicacidsodiumsalt,double-strandedCAS号42424-50-0分子式C19H27N7O16P2分子量671.41EINECS号1308068-626-2相关类别医药中间体;中间体;兽用鉰料添加剂和婴幼儿奶粉添加;医药兽药;原料药;生化试剂-聚合物类;医药原料;化学试剂;BiochemicalsandReagents;Biomaterials;CellCulture;MaterialsScience;Nucleosides;Nucleotides;OligonucleotidePolymersandCopolymers;Oligonucleotides;Otheradjuvants;SyntheticOligonucleotidesandRelatedReagents;VaccineAdjuvants;VaccineProductionMol文件42424-50-0.mol结构式双链聚肌胞性质储存条件−20°C溶解度H2O：可溶，10mg/mL形态冻干粉颜色白色至米白色水溶解性Water:10mg/mL(Needultrasonicandwarming)双链聚肌胞用途与合成方法生物活性Polyinosinic-polycytidylicacidsodium(Poly(I:C)sodium)是双链RNA的合成类似物，是一种TLR3和视黄酸诱导型基因I受体(RIG-I和MDA5)的激动剂。Polyinosinic-polycytidylicacidsodium可以用作疫苗佐剂，以增强先天性和适应性免疫反应，并改变肿瘤的微环境，还可以直接触发癌细胞发生凋亡。靶点TLR3,RIG-I,MDA5,apoptosis体外研究Polyinosinic-polycytidylicacid(20ng/mL;24hours;WM793,WM278,WM239A,WM9and1205Lucells)treatmentstronglyreducesviabilityfrom100%incontrolsto20%–50%within24hours.Polyinosinic-polycytidylicacid(200ng/mL;24hours;1205Lucells)treatmentinducesapoptosisin1205Lucells.Polyinosinic-polycytChemicalbookidylicacid(3ng/mL;24hours;1205Lucells)treatmentinducesIFN-βexpressioninmelanomacells.SilencingofRIG-IandMDA-5confirmedthatinductionofIFN-βbyPolyinosinic-polycytidylicacidrequiredRIG-IandMDA-5,respectively,andthatrequiredIPS-1.Polyinosinic-polycytidylicacid(5ng/mL;24hours;1205Lucells)treatmentrevealsactivesubunitsofcaspase-9andcaspase-8inmelanomacells.Polyinosinic-polycytidylicacidsodiumispreparedforinjectionbyresuspendinginsterilesaline,heatingto50°Cataconcentrationof2mg/mltoensurecompletesolubilisationandthenallowingtocoolnaturallytoroomtemperaturetoensureproperannealingofdouble-strandedRNA.PolyI:Cisstoredat-20°Cuntiluse.CellViabilityAssayCellLine:WM793,WM278,WM239A,WM9and1205LucellsConcentration:20ng/mLIncubationTime:24hoursResult:Stronglyreducedviabilityfrom100%incontrolsto20%–50%within24hours.ApoptosisAnalysisCellLine:1205LucellsConcentration:200ng/mLIncubationTime:24hoursResult:Inducedapoptosisin1205Lucells.RT-PCRCellLine:1205LucellsConcentration:3ng/mLIncubationTime:24hoursResult:InducedIFN-βexpressioninmelanomacells.WesternBlotAnalysisCellLine:1205LucellsConcentration:5ng/mLIncubationTime:24hoursResult:Revealedactivesubunitsofcaspase-9andcaspase-8inmelanomacells.体内研究Polyinosinic-polycytidylicacidtreatmentinhibitstumorgrowthinNOD/SCIDimmunodeficientmiceinjectedwith1205Lucells.ThelevelofhumanDNAis50%lowerinmicetreatedwithPolyinosinic-polycytidylicacid.用途一种可作为在TLR3水平对细胞信号转导进行研究的模型RNA的双链均聚物。也是一种TRIF依赖性toll样受体-3(TLR3)配体。 |
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